Description
The strengths and limitations of DNA evidence in criminal cases is discussed and background scientific information needed to understand the basis of DNA profiling is provided. The lab includes the study of the DNA restriction enzyme function and uses electrophoresis to separate DNA sample fragments. Standard curves are constructed to make precise determinations of DNA fragment sizes. Students will also have the opportunity to read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the laboratory activity and use restriction digest maps of lambda bacteriophage genomes to design novel plasmids. Students learn how restriction enzymes function and how they are used in genetic engineering and discuss scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering